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Monthly Archives: January 2017

Cutting edge study on Roundup effect on liver.

Fatty Liver disease and glyphosate

Cutting-edge molecular profiling analyses reveal that the popular weedkiller Roundup causes liver damage at doses permitted by regulators. Report: Claire Robinson

The new study

Mesnage R, Renney G, Séralini GE, Ward M, Antoniou MN. Multiomics reveal non-alcoholic fatty liver disease in rats following chronic exposure to an ultra-low dose of Roundup herbicide. Scientific Reports, 2016; 6:39328. http://www.nature.com/articles/srep39328

This article highlights the process and results of this study.

The weedkiller Roundup causes non-alcoholic fatty liver disease at very low doses permitted by regulators worldwide, a new peer-reviewed study shows. The study is the first ever to show a causative link between consumption of Roundup at a real-world environmentally relevant dose and a serious disease.

The new peer-reviewed study, led by Dr Michael Antoniou at King’s College London, used cutting-edge profiling methods to describe the molecular composition of the livers of female rats fed an extremely low dose of Roundup weedkiller, which is based on the chemical glyphosate, over a 2-year period.

The dose of glyphosate from the Roundup administered was thousands of times below what is permitted by regulators worldwide.

The study revealed that these animals suffered from non-alcoholic fatty liver disease (NAFLD).

Dr Antoniou said: “The findings of our study are very worrying as they demonstrate for the first time a causative link between an environmentally relevant level of Roundup consumption over the long-term and a serious disease – namely non-alcoholic fatty liver disease.

“Our results also suggest that regulators should reconsider the safety evaluation of glyphosate-based herbicides.”

Potentially serious implications for human health

The new results demonstrate that long-term consumption of an ultra-low dose of Roundup at a glyphosate daily intake level of only 4 nanograms per kilogram of bodyweight per day, which is 75,000 times below EU and 437,500 below US permitted levels, results in NAFLD.

Regulators worldwide accept toxicity studies in rats as indicators of human health risks. So the results of this latest study have serious implications for human health.

NAFLD currently affects 25% of the US population and similar numbers of Europeans. Risk factors include being overweight or obese, having diabetes, or having high cholesterol or high triglycerides (a constituent of body fat) in the blood. However, some people develop NAFLD even if they do not have any of these known risk factors. The new study raises the question of whether exposure to Roundup is a hitherto unrecognized risk factor.

Symptoms of NAFLD include fatigue, weakness, weight loss, loss of appetite, nausea, abdominal pain, spider-like blood vessels, yellowing of the skin and eyes (jaundice), itching, fluid build-up and swelling of the legs and abdomen, and mental confusion.

Definitive confirmation of liver dysfunction from low dose of Roundup

In the new study the researchers undertook a followup protein composition profile (“proteomics”) and small molecule metabolite biochemical profile (“metabolomics”) investigation of the same liver samples to confirm the prediction of disease suggested by the transcriptomics gene expression profile analysis. As the proteomics and metabolomics directly measure the actual composition of the organ, these analytical methods provide a definitive assessment of its health or disease status.

Overall, metabolomics and proteomics disturbances showed a substantial overlap with biochemical hallmarks of NAFLD and its progression to steatohepatosis (serious fatty liver disease). Therefore they definitively confirm that serious liver disease has resulted from chronic ultra-low dose Roundup exposure.

The findings in detail

Proteins significantly disturbed (214 out of 1906 detected), as shown by the proteomics profiling, reflected a type of cell damage from reactive oxygen (peroxisomal proliferation), steatosis (serious fatty liver disease) and necrosis (areas of dead tissue).

The metabolomics analysis (55 metabolites altered out of 673 detected) confirmed lipotoxic (excess fatty tissue) conditions and oxidative stress. Metabolite alterations were also associated with hallmarks of serious liver toxicity.

NOT Substantially Equivalent ! In depth article of this new analysis.

GMO maize NK603 is not substantially equivalent to its non-GMO counterpart

A new analysis using cutting-edge molecular profiling methods shows the genetic engineering process caused large increases in two potentially toxic compounds in a Monsanto Roundup-tolerant maize. Report: Claire Robinson
A GMO maize variety that has been in the food and feed supply for years is not substantially equivalent to its non-GMO counterpart, the results of a new peer-reviewed study[1] show.

The study, published in Scientific Reports, also shows that the changes in the GMO maize resulted from the genetic engineering process and could have implications for the health of people and animals that eat the maize.

Yet these changes were not taken into account in the European Food Safety Authority’s (EFSA’s) risk assessment[2] – suggesting that a new evaluation must be performed on the basis of up-to-date science.

EFSA’s conclusion on NK603 is false

The basis of GMO approvals worldwide is the concept of substantial equivalence, meaning that the GMO maize is compositionally the same as the non-GMO counterpart (nearest relative).

In 2009 the EFSA GMO Panel concluded that “maize NK603 is compositionally equivalent to conventional maize”, except for the intended change – the presence of extra proteins that make the maize tolerant to glyphosate herbicides such as Roundup.

However, the new study, by a team of researchers led by Dr Michael Antoniou at King’s College London, shows that EFSA’s conclusion is false and that the genetic engineering process has had far-reaching unintended effects on the composition of NK603 maize.
Major differences caused by the GM process

The researchers’ in-depth analysis of the types of proteins (“proteomics”) and small biochemical molecules (“metabolomics”) revealed major differences between NK603 maize and its non-GMO counterpart.

A total of 117 proteins and 91 small molecule biochemicals (metabolites) were found to be significantly altered in NK603 corn by the GM transformation process. The GMO and non-GMO maize were grown in the same location and under the same conditions, ruling out the possibility that environmental factors such as the spraying of Roundup or different growing soils caused the differences.

The results of the analysis showed disturbances in energy utilisation and oxidative stress (damage to cells and tissues by reactive oxygen) in the GMO maize. There were also, in Dr Antoniou’s words, “worryingly large increases” in substances called polyamines.

Polyamines found to be present in increased amounts in GMO NK603 corn included putrescine and cadaverine.

To non-scientists, these substances might sound like something that oozes from dead bodies. And they wouldn’t be wrong. As Wikipedia tells us, “The two compounds are largely responsible for the foul odour of putrefying flesh” – as well as contributing to the smell of bad breath. Mmmm… lovely!

Although some polyamines may have beneficial effects in certain contexts, Dr Antoniou said that others, such as putrescine and cadaverine, can produce “various toxic effects. For example, they enhance the effects of histamine, thus heightening allergic reactions, and both have been implicated in the formation of carcinogenic substances called nitrosamines with nitrite in meat products.”

There is no indication in the EFSA assessment of NK603 maize that Monsanto measured these compounds in the crude compositional analysis it carried out to gain regulatory approval.
NK603 maize produced signs of toxic effects in rats

The actual maize tested in the new analysis was previously found to give rise to signs of toxic effects in rats, notably liver and kidney damage, in the Séralini 2-year feeding study.[3]

Could the increased levels of cadaverine and putrescine found in the NK603 maize samples account for these apparent health effects? Dr Antoniou said this question “needs to be further analysed in long-term animal feeding studies, using methods that specifically and more accurately quantify the amounts of these polyamines and their effects”.

However, what is certain is that the basis for regulatory approvals of NK603 maize looks decidedly shaky. As Dr Antoniou concluded, “Our study clearly shows that the GM transformation process results in profound compositional differences in NK603, demonstrating that this GMO maize is not substantially equivalent to its non-GMO counterpart. Our results call for a more thorough evaluation of the safety of NK603 maize consumption on a long-term basis.”

Notes

1. Mesnage R, Agapito-Tenfen S, Vilperte V, Renney G, Ward M, Séralini GE, Nodari N, Antoniou MN. An integrated multi-omics analysis of the NK603 Roundup-tolerant GM maize reveals metabolism disturbances caused by the transformation process. Scientific Reports, 2016; 6:37855. Open access: http://www.nature.com/articles/srep37855
2. EFSA. Applications (references EFSA-GMO-NL-2005-22, EFSA-GMO-RX-NK603) for the placing on the market of the genetically modified glyphosate tolerant maize NK603 for cultivation, food and feed uses, import and processing and for renewal of the authorisation of maize NK603 as existing products, both under Regulation (EC) No 1829/2003 from Monsanto: Scientific Opinion of the Panel on Genetically Modified Organisms (Questions No EFSA-Q-2005-249, No EFSA-Q-2008-075), Adopted on 27 May 2009. EFSA Journal 2009. http://onlinelibrary.wiley.com/doi/10.2903/j.efsa.2009.1137/pdf
3. Séralini et al. Republished study: long-term toxicity of a Roundup herbicide and a Roundup-tolerant genetically modified maize. Environmental Sciences Europe, 2014; 26(14). http://www.enveurope.com/content/26/1/14/abstract

Read this article on the GMWatch website here:
http://www.gmwatch.org/news/latest-news/17378

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